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American Society of Microbiology ABMM Sample Questions:

1. A patient who received a solid organ transplant develops a pneumonia. Bronchoalveolar lavage fluid is positive for a single-stranded RNA virus that can cause giant cell formation with intranuclear and intracytoplasmic inclusions. The MOST likely causative agent is:

A) Respiratory syncytial virus (RSV)
B) Adenovirus
C) Human metapneumovirus
D) Parainfluenza virus

2. A patient with a history of recurrent fungal skin infections presents with a scaly, erythematous rash on their feet. Microscopic examination of skin scrapings treated with potassium hydroxide (KOH) reveals septate hyphae and arthroconidia. The MOST likely genus of the dermatophyte is:

A) Trichophyton
B) Aspergillus
C) Candida
D) Malassezia

3. A clinical microbiology laboratory is validating a new multiplex PCR assay for the simultaneous detection of several gastrointestinal pathogens, including Salmonella, Shigella, and Campylobacter. To ensure the accuracy and reliability of the assay, it is crucial to include appropriate controls. Which of the following sets of controls would be MOST comprehensive?

A) Positive controls for each target organism, a negative control containing non-target DNA, and a process control to monitor nucleic acid extraction.
B) Positive controls for each target organism, a negative control without template DNA, an internal amplification control, and a process control to monitor nucleic acid extraction.
C) Positive controls for each target organism, a negative control without template DNA, and an internal amplification control.
D) Positive controls for each target organism and a negative control without any template DNA.

4. A clinical microbiology laboratory is performing quality control on antimicrobial susceptibility testing. The zone diameter for Escherichia coli ATCC 25922 tested against ciprofloxacin is consistently smaller than the acceptable range published by CLSI. The MOST likely cause of this discrepancy is:

A) Incubation at an incorrect temperature
B) All of the above
C) Inoculation with a bacterial suspension that is too dense
D) Use of expired antibiotic disks

5. A clinical microbiology laboratory is using flow cytometry to analyze lymphocyte subsets in a patient with suspected immunodeficiency. Which of the following cell surface markers is typically used to identify helper T cells?

A) CD19
B) CD4
C) CD56
D) CD8

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